Device

Part:BBa_K2170050:Design

Designed by: Max Mustermann   Group: iGEM16_LMU-TUM_Munich   (2016-10-11)


Secretory prokaryotic biotin binding receptor with enhanced monomeric avidin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2448
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 889


Keywords:


Abbreviations:

Design Notes

Related BioBrick:

  • Related BioBricks:

BBa_K2170052: Secretory prokaryotic biotin binding receptor with single chain avidin
BBa_K2170050: Secretory prokaryotic biotin binding receptor with enhanced monomeric avidin
Cloning details:

  • Designed in RFC10


Quality control measures:

  • Test digestion using EcoRI & PstI
  • Sequencing using primer VF2 & VR
  • Part was partly sequenced/Part was totally sequenced

Backbone:

  • Backbone name: pSB1C3
  • Resistance: Cp
  • Copynumber: high

Protein coding:

  • Protein: Secretory prokaryotic biotin presenting receptor with biotin acceptor peptide [Nucleotide 1030 to 2652]-->
  • Tag: internal A3C5 Tag

Enzymatic activity:

  • NanoLuc

Cytotoxicity:

  • none

Safety notes:
Known and anticipated sefety issues: none Known and anticipated security issues: none

Source

Source:

  • Preexisting BioBrick BBa_I739001; BBa_B0015; BBa_K1159001
  • Parts Synthesized by IDT
  • Professor Dr. Arne Skerra (Chair biological chemistry of TUM)


Organism:
Genesequence derived from Escherichia coli

  • Designed for the following Chassis: procaryotic cells


References

Literature references:

1. [http://jcb.rupress.org/content/108/2/229.short Kozak, M. (1989). The scanning model for translation: an update. The Journal of cell biology, 108(2), 229-241.]
2. [http://journals.plos.org/plosone/article?id=10.1371/journal.pone.000439 Kredel, S., Oswald, F., Nienhaus, K., Deuschle, K., Röcker, C., Wolff, M., ... & Wiedenmann, J. (2009). mRuby, a bright monomeric red fluorescent protein for labeling of subcellular structures. PloS one, 4(2), e4391.]
3. Bajar, B. T., Wang, E. S., Lam, A. J., Kim, B. B., Jacobs, C. L., Howe, E. S., ... & Chu, J. (2016). Improving brightness and photostability of green and red fluorescent proteins for live cell imaging and FRET reporting. Scientific reports, 6.
4. [http://www.nature.com/nprot/journal/v2/n6/abs/nprot.2007.209.html Schmidt, T. G., & Skerra, A. (2007). The Strep-tag system for one-step purification and high-affinity detection or capturing of proteins. Nature protocols, 2(6), 1528-1535.]

Database references: